| Registro completo |
| Provedor de dados: |
OAK
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| País: |
Japan
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| Título: |
Apical membrane antigen 1 is a cross-reactive antigen between Neospora caninum and Toxoplasma gondii, and the anti-NcAMA1 antibody inhibits host cell invasion by both parasites
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| Autores: |
Zhang, Houshuang
Compaore, Muller K.A.
Lee, Eung-goo
Liao, Min
Zhang, Guohong
Sugimoto, Chihiro
Fujisaki, Kozo
Nishikawa, Yoshifumi
Xuan, Xuenan
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| Data: |
2007-02
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| Ano: |
2007
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| Palavras-chave: |
Neospora caninum
Toxoplasma gondii
Apical membrane antigen 1
Cross-reactive
Invasion
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| Resumo: |
The cross-reactive antigens of Neospora caninum and Toxoplasma gondii are important in the exploration to determine the common mechanisms of parasite-host interaction. In this study, a gene encoding N. caninum apical membrane antigen 1 (NcAMA1) was identified by immunoscreening of a N. caninum tachyzoite cDNA expression library with antisera. from mice immunized with recombinant T gondii apical membrane antigen 1 (TgAMA 1). NcAMA1 was encoded by an open reading frame of 1695 bp, which encoded a protein of 564 amino acids. The single-copy NcAMA1 gene was interrupted by seven introns. NcAMA1 showed 73.6% amino acid identity to TgAMA1. Mouse polyclonal antibodies raised against the recombinant NcAMA1 (rNcAMA1) recognized a 69-kDa native parasite protein by Western blotting. Immunofluorescence analysis showed that NcAMA1 was localized to the apical end of tachyzoites. Two-dimensional electrophoresis and Western blotting indicated that an approximately 57-kDa cleavage product was released into the excretory/secretory products of N. caninum. Preincubation of free tachyzoites with anti-rNcAMA1 IgG antibodies inhibited the invasion into host cells by N. caninum and T gondii. These results indicated that AMA1 is a cross-reactive antigen between N. caninum and T gondii and a potential common vaccine candidate to control two parasites.
http://www.sciencedirect.com/science/journal/01666851
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| Idioma: |
Inglês
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| Identificador: |
http://ir.obihiro.ac.jp/dspace/handle/10322/1034
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| Editor: |
Elsevier
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| Formato: |
application/pdf
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